Billing and Coding: Vitamin D Assay Testing

This billing and coding guideline complements the LCD for vitamin D assays and requires accurate ICD-10 coding, ordering physician name/NPI when applicable, and medical-record justification of the selected test. Procedure codes may be subject to NCCI/OPPS edits, composite sources of 25‑OH vitamin D should not be billed separately, and repeat testing for levels 20–50 ng/mL in clinically stable patients requires documented medical necessity.

"Procedure codes for vitamin D assays may be subject to National Correct Coding Initiative (NCCI) edits or OPPS packaging edits; verify NCCI and OPPS requirements prior to billing Medicare."

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